Cargando...
Rapidly evolving homing CRISPR barcodes
We present here an approach for engineering evolving DNA barcodes in living cells. The methodology entails using a homing guide RNA (hgRNA) scaffold that directs the Cas9-hgRNA complex to target the DNA locus of the hgRNA itself. We show that this homing CRISPR-Cas9 system acts as an expressed genet...
Guardado en:
| Publicado en: | Nat Methods |
|---|---|
| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2016
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5322472/ https://ncbi.nlm.nih.gov/pubmed/27918539 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4108 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|