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High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system

BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the f...

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Bibliografiset tiedot
Julkaisussa:Microb Cell Fact
Päätekijät: Zhang, Kang, Su, Lingqia, Duan, Xuguo, Liu, Lina, Wu, Jing
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BioMed Central 2017
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5319110/
https://ncbi.nlm.nih.gov/pubmed/28219382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0649-1
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