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High-level extracellular protein production in Bacillus subtilis using an optimized dual-promoter expression system
BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the f...
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| Publicado no: | Microb Cell Fact |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5319110/ https://ncbi.nlm.nih.gov/pubmed/28219382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0649-1 |
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