Primary cell culture of live neurosurgically-resected aged adult human brain cells and single cell transcriptomics

Investigation of human CNS disease and drug effects has been hampered by the lack of a system that enables single cell analysis on live adult patient brain cells. We developed a culturing system, based on a papain-aided procedure, for resected adult human brain tissue removed during neurosurgery. We...

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Publicado no:Cell Rep
Main Authors: Spaethling, Jennifer M., Na, Young-Ji, Lee, Jaehee, Ulyanova, Alexandra V., Baltuch, Gordon H., Bell, Thomas J., Brem, Steven, Chen, H. Isaac, Dueck, Hannah, Fisher, Stephen A., Garcia, Marcela P., Khaladkar, Mugdha, Kung, David K., Lucas, Timothy H., O’Rourke, Donald M., Stefanik, Derek, Wang, Jinhui, Wolf, John A., Bartfai, Tamas, Grady, M. Sean, Sul, Jai-Yoon, Kim, Junhyong, Eberwine, James H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5316103/
https://ncbi.nlm.nih.gov/pubmed/28099855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2016.12.066
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