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An optimized N(pro)-based method for the expression and purification of intrinsically disordered proteins for an NMR study

Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in performing X-ray crystallography for IDPs, nuclear magnetic resonance (NMR) spectroscopy is the first choic...

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Detalles Bibliográficos
Publicado en:Intrinsically Disord Proteins
Main Authors: Goda, Natsuko, Matsuo, Naoki, Tenno, Takeshi, Ishino, Sonoko, Ishino, Yoshizumi, Fukuchi, Satoshi, Ota, Motonori, Hiroaki, Hidekazu
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5314886/
https://ncbi.nlm.nih.gov/pubmed/28232886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21690707.2015.1011004
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