An optimized N(pro)-based method for the expression and purification of intrinsically disordered proteins for an NMR study

Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in performing X-ray crystallography for IDPs, nuclear magnetic resonance (NMR) spectroscopy is the first choic...

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Udgivet i:Intrinsically Disord Proteins
Main Authors: Goda, Natsuko, Matsuo, Naoki, Tenno, Takeshi, Ishino, Sonoko, Ishino, Yoshizumi, Fukuchi, Satoshi, Ota, Motonori, Hiroaki, Hidekazu
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2015
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5314886/
https://ncbi.nlm.nih.gov/pubmed/28232886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21690707.2015.1011004
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