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An optimized N(pro)-based method for the expression and purification of intrinsically disordered proteins for an NMR study
Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in performing X-ray crystallography for IDPs, nuclear magnetic resonance (NMR) spectroscopy is the first choic...
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| Pubblicato in: | Intrinsically Disord Proteins |
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| Autori principali: | , , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Taylor & Francis
2015
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5314886/ https://ncbi.nlm.nih.gov/pubmed/28232886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21690707.2015.1011004 |
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