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Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells

Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentiall...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Gong, Haixia, Liu, Menglin, Klomp, Jeff, Merrill, Bradley J., Rehman, Jalees, Malik, Asrar B.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5309830/
https://ncbi.nlm.nih.gov/pubmed/28198371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep42127
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