Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters

BACKGROUND: Traditional approaches for generating goat pluripotent stem cells (iPSCs) suffer from complexity and low preparation efficiency. Therefore, we tried to derive goat iPSCs with a new method by transfecting exogenous Oct4, Sox2, Klf4 and c-Myc mRNAs into goat embryonic fibroblasts (GEFs), a...

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Dettagli Bibliografici
Pubblicato in:BMC Biotechnol
Autori principali: Chen, Hao, Zuo, Qisheng, Wang, Yingjie, Song, Jiuzhou, Yang, Huilin, Zhang, Yani, Li, Bichun
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2017
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5307868/
https://ncbi.nlm.nih.gov/pubmed/28193206
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-017-0336-7
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