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Engineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency

Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocoli...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Niu, Canfang, Yang, Peilong, Luo, Huiying, Huang, Huoqing, Wang, Yaru, Yao, Bin
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5301473/
https://ncbi.nlm.nih.gov/pubmed/28186144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep42133
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