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Engineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency
Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocoli...
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Yayımlandı: | Sci Rep |
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Asıl Yazarlar: | , , , , , |
Materyal Türü: | Artigo |
Dil: | Inglês |
Baskı/Yayın Bilgisi: |
Nature Publishing Group
2017
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Konular: | |
Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5301473/ https://ncbi.nlm.nih.gov/pubmed/28186144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep42133 |
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