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Engineering the residual side chains of HAP phytases to improve their pepsin resistance and catalytic efficiency
Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocoli...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5301473/ https://ncbi.nlm.nih.gov/pubmed/28186144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep42133 |
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