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Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening
The Golden Gate strategy entails the use of type IIS restriction enzymes, which cut outside of their recognition sequence. It enables unrestricted design of unique DNA fragments that can be readily and seamlessly recombined. Successfully employed in other synthetic biology applications, we demonstra...
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| Publicado no: | PLoS One |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5298319/ https://ncbi.nlm.nih.gov/pubmed/28178357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0171741 |
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