A touchdown nucleic acid amplification protocol as an alternative to culture backup for immunofluorescence in the routine diagnosis of acute viral respiratory tract infections

BACKGROUND: Immunofluorescence and virus culture are the main methods used to diagnose acute respiratory virus infections. Diagnosing these infections using nucleic acid amplification presents technical challenges, one of which is facilitating the different optimal annealing temperatures needed for...

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Vydáno v:	BMC Microbiol
Hlavní autoři:	Coyle, Peter V, Ong, Grace M, O'Neill, Hugh J, McCaughey, Conall, De Ornellas, Dennis, Mitchell, Frederick, Mitchell, Suzanne J, Feeney, Susan A, Wyatt, Dorothy E, Forde, Marian, Stockton, Joanne
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2004
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC529439/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15504232/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2180-4-41
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