A touchdown nucleic acid amplification protocol as an alternative to culture backup for immunofluorescence in the routine diagnosis of acute viral respiratory tract infections

BACKGROUND: Immunofluorescence and virus culture are the main methods used to diagnose acute respiratory virus infections. Diagnosing these infections using nucleic acid amplification presents technical challenges, one of which is facilitating the different optimal annealing temperatures needed for...

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Detalhes bibliográficos
Main Authors: Coyle, Peter V, Ong, Grace M, O'Neill, Hugh J, McCaughey, Conall, De Ornellas, Dennis, Mitchell, Frederick, Mitchell, Suzanne J, Feeney, Susan A, Wyatt, Dorothy E, Forde, Marian, Stockton, Joanne
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2004
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC529439/
https://ncbi.nlm.nih.gov/pubmed/15504232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-4-41
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