Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads

This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompet...

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Detalhes bibliográficos
Publicado no:Biomicrofluidics
Main Authors: Moore, J. A., Nemat-Gorgani, M., Madison, A. C., Sandahl, M. A., Punnamaraju, S., Eckhardt, A. E., Pollack, M. G., Vigneault, F., Church, G. M., Fair, R. B., Horowitz, M. A., Griffin, P. B.
Formato: Artigo
Idioma:Inglês
Publicado em: AIP Publishing LLC 2017
Assuntos:
Regular Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5291792/
https://ncbi.nlm.nih.gov/pubmed/28191268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4975391
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