Automated electrotransformation of Escherichia coli on a digital microfluidic platform using bioactivated magnetic beads

This paper reports on the use of a digital microfluidic platform to perform multiplex automated genetic engineering (MAGE) cycles on droplets containing Escherichia coli cells. Bioactivated magnetic beads were employed for cell binding, washing, and media exchange in the preparation of electrocompet...

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Bibliografiske detaljer
Udgivet i:Biomicrofluidics
Main Authors: Moore, J. A., Nemat-Gorgani, M., Madison, A. C., Sandahl, M. A., Punnamaraju, S., Eckhardt, A. E., Pollack, M. G., Vigneault, F., Church, G. M., Fair, R. B., Horowitz, M. A., Griffin, P. B.
Format: Artigo
Sprog:Inglês
Udgivet: AIP Publishing LLC 2017
Fag:
Regular Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5291792/
https://ncbi.nlm.nih.gov/pubmed/28191268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4975391
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