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Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7

ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and in vitro assays. The protein expressed in an RNase H-deficient mutant Escherichia coli strain functio...

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Autors principals: Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC528802/
https://ncbi.nlm.nih.gov/pubmed/15520465
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh917
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