Stabilization by Fusion to the C-terminus of Hyperthermophile Sulfolobus tokodaii RNase HI: A Possibility of Protein Stabilization Tag

RNase HI from the hyperthermophile Sulfolobus tokodaii (Sto-RNase HI) is stabilized by its C-terminal residues. In this work, the stabilization effect of the Sto-RNase HI C-terminal residues was investigated in detail by thermodynamic measurements of the stability of variants lacking the disulfide b...

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Detalhes bibliográficos
Main Authors: Takano, Kazufumi, Okamoto, Tomohiro, Okada, Jun, Tanaka, Shun-ichi, Angkawidjaja, Clement, Koga, Yuichi, Kanaya, Shigenori
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3023800/
https://ncbi.nlm.nih.gov/pubmed/21283826
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0016226
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