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Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
[Image: see text] Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (K(d)) for protein–peptide interactions can be inferred. With the current platform, four...
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| Publicado no: | Anal Chem |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American
Chemical
Society
2017
|
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5287478/ https://ncbi.nlm.nih.gov/pubmed/28192993 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b02528 |
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