Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.

U7 snRNP is a low-abundance small nuclear ribonucleoprotein particle essential for 3' processing of replication-dependent histone pre-mRNA. We have developed a two-step purification of the particle from TB21 mouse mastocytoma cell nuclear extracts, with about a 20% overall yield, using affinity...

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Autores principales: Smith, H O, Tabiti, K, Schaffner, G, Soldati, D, Albrecht, U, Birnstiel, M L
Formato: Artigo
Lenguaje:Inglês
Publicado: 1991
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC52805/
https://ncbi.nlm.nih.gov/pubmed/1835087
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