Two-step affinity purification of U7 small nuclear ribonucleoprotein particles using complementary biotinylated 2'-O-methyl oligoribonucleotides.

U7 snRNP is a low-abundance small nuclear ribonucleoprotein particle essential for 3' processing of replication-dependent histone pre-mRNA. We have developed a two-step purification of the particle from TB21 mouse mastocytoma cell nuclear extracts, with about a 20% overall yield, using affinity...

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Hauptverfasser: Smith, H O, Tabiti, K, Schaffner, G, Soldati, D, Albrecht, U, Birnstiel, M L
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC52805/
https://ncbi.nlm.nih.gov/pubmed/1835087
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