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Intramolecular transposition by a synthetic IS50 (Tn5) derivative.

We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance gene...

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Detalles Bibliográficos
Main Authors: Tomcsanyi, T, Berg, C M, Phadnis, S H, Berg, D E
Formato: Artigo
Idioma:Inglês
Publicado: 1990
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC526819/
https://ncbi.nlm.nih.gov/pubmed/2172212
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