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Probing the Role of the Backbone Carbonyl Interaction with the Cu(A) Center in Azurin by Replacing the Peptide Bond with an Ester Linkage

The role of a backbone carbonyl interaction with an engineered Cu(A) center in azurin was investigated by developing a method of synthesis and incorporation of a depsipeptide where one of the amide bonds in azurin is replaced by an ester bond using expressed protein ligation. Studies by electronic a...

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Pubblicato in:Chem Commun (Camb)
Autori principali: Clark, Kevin M., Tian, Shiliang, van der Donk, Wilfred A., Lu, Yi
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5253137/
https://ncbi.nlm.nih.gov/pubmed/27918029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6cc07274g
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