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Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High Sensitivity
A real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to reach a sensitivity of 0.0125 parasites/ml of blood. In order to analyze the incidence of heterogeneity and number of minicircles, we performed comparative PCR by using the Leishmania DNA polymerase gene a...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2004
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC525214/ https://ncbi.nlm.nih.gov/pubmed/15528722 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.11.5249-5255.2004 |
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