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Quantification of Leishmania infantum DNA by a Real-Time PCR Assay with High Sensitivity

A real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to reach a sensitivity of 0.0125 parasites/ml of blood. In order to analyze the incidence of heterogeneity and number of minicircles, we performed comparative PCR by using the Leishmania DNA polymerase gene a...

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Detalhes bibliográficos
Main Authors: Mary, Charles, Faraut, Françoise, Lascombe, Laurie, Dumon, Henri
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC525214/
https://ncbi.nlm.nih.gov/pubmed/15528722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.11.5249-5255.2004
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