Structural determinants of stereospecificity in yeast alcohol dehydrogenase.

Replacing Leu-182 by Ala in yeast alcohol dehydrogenase (YADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) yields a mutant that retains 34% of its kcat value and makes one stereochemical "mistake" every 850,000 turnovers (instead of approximately 1 error every 7,000,000,000 turnovers in nativ...

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Hauptverfasser: Weinhold, E G, Glasfeld, A, Ellington, A D, Benner, S A
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC52520/
https://ncbi.nlm.nih.gov/pubmed/1924300
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