Structural determinants of stereospecificity in yeast alcohol dehydrogenase.

Replacing Leu-182 by Ala in yeast alcohol dehydrogenase (YADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) yields a mutant that retains 34% of its kcat value and makes one stereochemical "mistake" every 850,000 turnovers (instead of approximately 1 error every 7,000,000,000 turnovers in native YADH) in...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Weinhold, E G, Glasfeld, A, Ellington, A D, Benner, S A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52520/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1924300/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.19.8420
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