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Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster

To exploit the huge potential of whole-genome sequence information, the ability to efficiently synthesize long, accurate DNA sequences is becoming increasingly important. An approach proposed toward this end involves the synthesis of ≈5-kb segments of DNA, followed by their assembly into longer sequ...

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Detalhes bibliográficos
Main Authors: Kodumal, Sarah J., Patel, Kedar G., Reid, Ralph, Menzella, Hugo G., Welch, Mark, Santi, Daniel V.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC524854/
https://ncbi.nlm.nih.gov/pubmed/15496466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0406911101
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