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Improved Affinity of a Human Anti-Entamoeba histolytica Gal/GalNAc Lectin Fab Fragment by a Single Amino Acid Modification of the Light Chain
We previously produced, in Escherichia coli, a human monoclonal antibody Fab fragment, CP33, specific for the galactose- and N-acetyl-d-galactosamine-inhibitable lectin of Entamoeba histolytica. To prepare antibodies with a higher affinity to the lectin, recombination PCR was used to exchange Ser(91...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology
2004
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC524788/ https://ncbi.nlm.nih.gov/pubmed/15539510 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.11.6.1085-1088.2004 |
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