Large-Scale Mutagenesis of the Yeast Genome Using a Tn7-Derived Multipurpose Transposon

We present here an unbiased and extremely versatile insertional library of yeast genomic DNA generated by in vitro mutagenesis with a multipurpose element derived from the bacterial transposon Tn7. This mini-Tn7 element has been engineered such that a single insertion can be used to generate a lacZ...

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Main Authors: Kumar, Anuj, Seringhaus, Michael, Biery, Matthew C., Sarnovsky, Robert J., Umansky, Lara, Piccirillo, Stacy, Heidtman, Matthew, Cheung, Kei-Hoi, Dobry, Craig J., Gerstein, Mark B., Craig, Nancy L., Snyder, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC524422/
https://ncbi.nlm.nih.gov/pubmed/15466296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2875304
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