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GTP/GDP exchange by Sec12p enables COPII vesicle bud formation on synthetic liposomes
The generation of COPII vesicles from synthetic liposome membranes requires the minimum coat components Sar1p, Sec23/24p, Sec13/31p, and a nonhydrolyzable GTP analog such as GMP-PNP. However, in the presence of GTP and the full complement of coat subunits, nucleotide hydrolysis by Sar1p renders the...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
European Molecular Biology Organization
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC524392/ https://ncbi.nlm.nih.gov/pubmed/15457212 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7600428 |
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