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High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs.

With the goal of developing techniques for DNA insertional mutagenesis in zebrafish, we established procedures for rapidly obtaining and injecting large numbers of fertilized eggs. Using either of two plasmid constructs, we injected uncut DNA into fertilized eggs at the one- or two-cell stage. Fish...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Culp, P, Nüsslein-Volhard, C, Hopkins, N
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52423/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1910170/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.18.7953
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