High-Throughput Selection of Retrovirus Producer Cell Lines Leads to Markedly Improved Efficiency of Germ Line-Transmissible Insertions in Zebra Fish

Vesicular stomatitis virus glycoprotein G-pseudotyped mouse retroviral vectors have been used as mutagens for a large-scale insertional mutagenesis screen in the zebra fish. To reproducibly generate high-titer virus stocks, we devised a method for rapidly selecting cell lines that can yield high-tit...

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Bibliographic Details
Main Authors: Chen, Wenbiao, Burgess, Shawn, Golling, Greg, Amsterdam, Adam, Hopkins, Nancy
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2002
Subjects:
Gene Therapy
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC135931/
https://ncbi.nlm.nih.gov/pubmed/11836396
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