Site-directed mutations of Dictyostelium actin: disruption of a negative charge cluster at the N terminus.
Aspartic acid residues in the N-terminal negative charge cluster of Dictyostelium actin were replaced with histidine residues by site-directed mutagenesis of the actin gene. The mutant actins were expressed in Dictyostelium cells and were purified to homogeneity by HPLC. Functional properties of the...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1991
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52372/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1831905/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.17.7711 |
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