Leukotriene A4 hydrolase: determination of the three zinc-binding ligands by site-directed mutagenesis and zinc analysis.

Three mutants of recombinant mouse leukotriene A4 (LTA4) hydrolase (3.3.2.6) were produced by site-directed mutagenesis on cDNA. The codons corresponding to His-295, His-299, or Glu-318 were replaced by codons encoding tyrosine, tyrosine, and glutamine, respectively. The mutated cDNAs were expressed...

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Detalhes bibliográficos
Main Authors: Medina, J F, Wetterholm, A, Rådmark, O, Shapiro, R, Haeggström, J Z, Vallee, B L, Samuelsson, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC52353/
https://ncbi.nlm.nih.gov/pubmed/1881903
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