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Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker

We developed an assay method for measuring dihydroorotate dehydrogenase (DHODH) activity in cultured HeLa cells and fibroblasts, and in stage III stomach cancer and adjacent normal tissues from the same patient. The assay comprised enzymatic reaction of DHODH with a large amount of dihydroorotic aci...

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Enregistré dans:
Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Yin, Sheng, Kabashima, Tsutomu, Zhu, Qinchang, Shibata, Takayuki, Kai, Masaaki
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5233952/
https://ncbi.nlm.nih.gov/pubmed/28084471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40670
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