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Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker

We developed an assay method for measuring dihydroorotate dehydrogenase (DHODH) activity in cultured HeLa cells and fibroblasts, and in stage III stomach cancer and adjacent normal tissues from the same patient. The assay comprised enzymatic reaction of DHODH with a large amount of dihydroorotic aci...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Yin, Sheng, Kabashima, Tsutomu, Zhu, Qinchang, Shibata, Takayuki, Kai, Masaaki
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5233952/
https://ncbi.nlm.nih.gov/pubmed/28084471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40670
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