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Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker

We developed an assay method for measuring dihydroorotate dehydrogenase (DHODH) activity in cultured HeLa cells and fibroblasts, and in stage III stomach cancer and adjacent normal tissues from the same patient. The assay comprised enzymatic reaction of DHODH with a large amount of dihydroorotic aci...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Yin, Sheng, Kabashima, Tsutomu, Zhu, Qinchang, Shibata, Takayuki, Kai, Masaaki
Format: Artigo
Language:Inglês
Published: Nature Publishing Group 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5233952/
https://ncbi.nlm.nih.gov/pubmed/28084471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep40670
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