Construction of an Enterococcus faecalis Tn917-Mediated-Gene-Disruption Library Offers Insight into Tn917 Insertion Patterns

Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequ...

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Pubblicato in:	J Bacteriol
Autori principali:	Garsin, Danielle A., Urbach, Jonathan, Huguet-Tapia, Jose C., Peters, Joseph E., Ausubel, Frederick M.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2004
Soggetti:	Bacteriophages, Transposons, and Plasmids
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC523214/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15489440/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.186.21.7280-7289.2004
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