Construction of an Enterococcus faecalis Tn917-Mediated-Gene-Disruption Library Offers Insight into Tn917 Insertion Patterns

Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequ...

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Библиографические подробности
Главные авторы: Garsin, Danielle A., Urbach, Jonathan, Huguet-Tapia, Jose C., Peters, Joseph E., Ausubel, Frederick M.
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2004
Предметы:
Bacteriophages, Transposons, and Plasmids
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC523214/
https://ncbi.nlm.nih.gov/pubmed/15489440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.21.7280-7289.2004
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