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Detection and Differentiation of Leishmania spp. in Clinical Specimens by Use of a SYBR Green-Based Real-Time PCR Assay

Leishmaniasis in humans is caused by Leishmania spp. in the subgenera Leishmania and Viannia. Species identification often has clinical relevance. Until recently, our laboratory relied on conventional PCR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequenc...

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Foilsithe in:J Clin Microbiol
Main Authors: de Almeida, Marcos E., Koru, Ozgur, Steurer, Francis, Herwaldt, Barbara L., da Silva, Alexandre J.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5228241/
https://ncbi.nlm.nih.gov/pubmed/27847378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01764-16
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