Detection of Alicyclobacillus spp. in Fruit Juice by Combination of Immunomagnetic Separation and a SYBR Green I Real-Time PCR Assay

An approach based on immunomagnetic separation (IMS) and SYBR Green I real-time PCR (real-time PCR) with species-specific primers and melting curve analysis was proposed as a rapid and effective method for detecting Alicyclobacillus spp. in fruit juices. Specific primers targeting the 16S rDNA seque...

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Bibliographic Details
Published in:PLoS One
Main Authors: Cai, Rui, Wang, Zhouli, Yuan, Yahong, Liu, Bin, Wang, Ling, Yue, Tianli
Format: Artigo
Language:Inglês
Published: Public Library of Science 2015
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4619346/
https://ncbi.nlm.nih.gov/pubmed/26488469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0141049
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