Development and Validation of a New Reliable Method for the Diagnosis of Avian Botulism

Liver is a reliable matrix for laboratory confirmation of avian botulism using real-time PCR. Here, we developed, optimized, and validated the analytical steps preceding PCR to maximize the detection of Clostridium botulinum group III in avian liver. These pre-PCR steps included enrichment incubatio...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Le Maréchal, Caroline, Rouxel, Sandra, Ballan, Valentine, Houard, Emmanuelle, Poezevara, Typhaine, Bayon-Auboyer, Marie-Hélène, Souillard, Rozenn, Morvan, Hervé, Baudouard, Marie-Agnès, Woudstra, Cédric, Mazuet, Christelle, Le Bouquin, Sophie, Fach, Patrick, Popoff, Michel, Chemaly, Marianne
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226734/
https://ncbi.nlm.nih.gov/pubmed/28076405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0169640
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