Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits

Abstract Objectives Few studies have tested DNA extraction methods to optimize the detection of Clostridium botulinum in environmental samples that can be collected during animal botulism outbreaks. In this study, we evaluated four commercial DNA extraction kits for the detection of C. botulinum gro...

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Detalhes bibliográficos
Main Authors: Caroline Le Maréchal, Sarah Fourour, Valentine Ballan, Sandra Rouxel, Rozenn Souillard, Marianne Chemaly
Formato: Artigo
Idioma:Inglês
Publicado em: BMC 2018-07-01
Colecção:BMC Research Notes
Assuntos:
Clostridium botulinum
DNA extraction
Real-time PCR
Detection
Freezing
Acesso em linha:http://link.springer.com/article/10.1186/s13104-018-3549-5
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