Analysis of Simian Immunodeficiency Virus-specific CD8(+) T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Peptide-major histocompatibility complex class I (pMHC-I) tetramers have been an invaluable tool to study CD8(+) T-cell responses. Because these reagents directly bind to T-cell receptors on the surface of CD8(+) T-lymphocytes, fluorochrome-labeled pMHC-I tetramers enable the accurate detection of a...

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Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Gonzalez-Nieto, Lucas, Domingues, Aline, Ricciardi, Michael, Gutman, Martin J., Maxwell, Helen S., Pedreño-Lopez, Nuria, Bailey, Varian, Magnani, Diogo M., Martins, Mauricio A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2016
Soggetti:
Immunology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226457/
https://ncbi.nlm.nih.gov/pubmed/28060314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54881
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