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Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
There are currently many chemical tools available to introduce chemical probes into proteins to study their structure and function. A useful method is protein conjugation by genetically introducing an unnatural amino acid containing a bioorthogonal functional group. This report describes a detailed...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5226445/ https://ncbi.nlm.nih.gov/pubmed/28060353 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54922 |
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