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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

We describe methods for studying the giant transcriptionally active lampbrush chromosomes (LBCs) found in the oocyte, or unlaid egg, of frogs and salamanders. Individual LBCs can be up to 1 mm in length and they reside in a gigantic nucleus, itself up to 0.5 mm in diameter. The large size of the chr...

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書誌詳細
出版年:J Vis Exp
主要な著者: Gall, Joseph G., Nizami, Zehra F.
フォーマット: Artigo
言語:Inglês
出版事項: MyJove Corporation 2016
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226360/
https://ncbi.nlm.nih.gov/pubmed/28060282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54103
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