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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
We describe methods for studying the giant transcriptionally active lampbrush chromosomes (LBCs) found in the oocyte, or unlaid egg, of frogs and salamanders. Individual LBCs can be up to 1 mm in length and they reside in a gigantic nucleus, itself up to 0.5 mm in diameter. The large size of the chr...
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Publicado no: | J Vis Exp |
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Main Authors: | , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5226360/ https://ncbi.nlm.nih.gov/pubmed/28060282 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54103 |
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