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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Electrophoretic Mobility Shift Assays (EMSA) are an instrumental tool to characterize the interactions between proteins and their target DNA sequences. Radioactivity has been the predominant method of DNA labeling in EMSAs. However, recent advances in fluorescent dyes and scanning methods have promp...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hsieh, Yi-Wen, Alqadah, Amel, Chuang, Chiou-Fen
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226320/
https://ncbi.nlm.nih.gov/pubmed/27929467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54863
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