Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Activity-based protein profiling (ABPP) is a method for the identification of an enzyme of interest in a complex proteome through the use of a chemical probe that targets the enzyme's active sites. A reporter tag introduced into the probe allows for the detection of the labeled enzyme by in-gel...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Romeo, Elisa, Pontis, Silvia, Ponzano, Stefano, Bonezzi, Fabiola, Migliore, Marco, Di Martino, Simona, Summa, Maria, Piomelli, Daniele
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5226263/
https://ncbi.nlm.nih.gov/pubmed/27911411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54652
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