Double targeted gene replacement for creating null mutants.

We have used double gene targeting to create homozygous gene replacements in the protozoan parasite Leishmania major, an asexual diploid. This method uses two independent selectable markers in successive rounds of gene targeting to replace both alleles of an endogenous gene. We developed an improved...

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Bibliografiske detaljer
Main Authors: Cruz, A, Coburn, C M, Beverley, S M
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC52255/
https://ncbi.nlm.nih.gov/pubmed/1651496
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