Double targeted gene replacement for creating null mutants.
We have used double gene targeting to create homozygous gene replacements in the protozoan parasite Leishmania major, an asexual diploid. This method uses two independent selectable markers in successive rounds of gene targeting to replace both alleles of an endogenous gene. We developed an improved...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52255/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1651496/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.16.7170 |
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